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Statistical analyses All data calculations and statistical analyses were performed using the R software ( version 4.2.2)

On the other hand, Fe 2+ and Fe 3+ are also cofactors that can enhance the activities of various metabolic enzymes, resulting in the formation of free radicals such as alkoxy (RO) and peroxy (RO 2 ), promoting lipid peroxidation, and inducing ferroptosis ( 1 ) and iron regulatory protein 2 (IRP 2 ) regulate intracellular iron storage, release, entry, and exit, thereby maintaining cellular iron homeostasis

Nat Commun 7:10498 Escudero-Esparza A, Jiang WG, Martin TA (2011) The Claudin family and its role in cancer and metastasis

The mixture was stirred vigorously for 15 min and aged for 30 min at 70 C and then the reaction mixture was cooled down to room temperature